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KMID : 0616120110420030282
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Korean Journal of Pharmacognosy
2011 Volume.42 No. 3 p.282 ~ p.287
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Anti-cancer Activity of Human Colon Cancer (HT-29) Cell Line from Different Fraction of Zanthoxylum schnifolium Fruits
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Han Woong
Hu Weicheng
Lee Young-Mee
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Abstract
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This study was presented to the anti-cancer activity from different fraction of Zanthoxylum schnifolium fruits. The values for human colon cancer cell(HT-29) survival rate of 0.3 mg/mL of 70% EtOH and 70% MeOH ethyl acetate fraction extracts were 7.620.173%, 7.660.037%, respectively. It was shown that human colon cancer cell(HT-29) survival rate was in a dose-dependent manner. The percentages of cells were increased in the sub-G0 and G0/G1 phase region, meaning that cell proliferation was decreased. The RT-PCR demonstrated that 70% EtOH and 70% MeOH ethyl acetate fraction extracts were down-regulated the expression of Bcl-2 and survivin genes in HT-29 cells. We examined that 70% EtOH and 70% MeOH ethyl acetate fraction extracts anti-cancer activities initiated through ROS generation suggesting that HT-29 cells treated with ethyl acetate fraction extracts induced ROS generation. Our results revealed that the Zanthoxylum schnifolium fruit may expect for anti-cancer activities in HT-29 cells.
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KEYWORD
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Anti-cancer, ROS, HT-29 cells, Human colon cancer cells, Zanthoxylum schnifolium
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